Using DNA Probes
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چکیده
منابع مشابه
Molecular identification of agrobacterium tumefaciens containing pCAMBIA 1305.2 plasmid using multiplex PCR and Gold nanoparticles multiplex probe
Conventional microbiology methods used to detect bacteria include multiple cultures and identification processes, so the results of lab work are painstaking and time-consuming. In recent years, more and more tend to use the diagnostic tests which are based on DNA; hence, DNA diagnostic biosensors have been created to perform DNA identification better. In this study, GUS and hpt genes were used ...
متن کاملMolecular identification of agrobacterium tumefaciens containing pCAMBIA 1305.2 plasmid using multiplex PCR and Gold nanoparticles multiplex probe
Conventional microbiology methods used to detect bacteria include multiple cultures and identification processes, so the results of lab work are painstaking and time-consuming. In recent years, more and more tend to use the diagnostic tests which are based on DNA; hence, DNA diagnostic biosensors have been created to perform DNA identification better. In this study, GUS and hpt genes were used ...
متن کاملGene Probe Designing for Evaluation of the Diversity of Bradyrhizobium japonicum Isolates
Many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. In this study for asessing genetic diversity among Bradyrhizobium japonicum isolates, two different probes (sucA and topA) chosen from the chromosomal genome of Bradyrhizobium strain USDA 110 were designed, evaluated by DNAMAN software and im...
متن کاملارزیابی کمی و بهینه سازی تشخیص DNA آسپرژیلوس فومیگاتوس در نمونه خون با استفاده از تکنیک Real time PCR
Background and purpose: Ïnvasive aspergillosis (ÏÂ) is a serious opportunistic infection caused by various species of Âspergillus in immnucompromissed individuals. Basically, rapid and early diagnosis prevents ÏÂ progression. The purpose of this study was to quantify and optimize detection of Âspergillus fumigatus DNÂ in blood samples using Real Time PÇR. Materials and methods: Five millil...
متن کاملQuantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...
متن کاملRole of ultrasound probes in transmission of hospital infections
Background: The ultrasonography probes are cleaned by absorbent soft, dry cloth. This question arose whether linear and convex ultrasound probes coupling with gel could perform as a means for nosocomial infections transmission, and which method is economical and more efficient for cleaning the probes. This study was conducted to answer these questions. Methods: One hundred – ninety two patients...
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تاریخ انتشار 1993